Journal: Frontiers in Molecular Neuroscience

Article Title: Circular RNA Expression Alteration and Bioinformatics Analysis in Rats After Traumatic Spinal Cord Injury

doi: 10.3389/fnmol.2018.00497

Figure Lengend Snippet: Comparison for candidate circRNAs expression in microarray and PCR.

Article Snippet: As shown in Supplementary Figure , the sample preparations, sample labeling, and microarray hybridizations were performed based on the manufacturer’s standard protocols (Arraystar).

Techniques: Comparison, Expressing, Microarray

Journal: Journal of Hematology & Oncology

Article Title: A novel lncRNA uc.134 represses hepatocellular carcinoma progression by inhibiting CUL4A-mediated ubiquitination of LATS1

doi: 10.1186/s13045-017-0449-4

Figure Lengend Snippet: LncRNA uc.134 inhibits the CUL4A-mediated ubiquitination of LATS1 to regulate Hippo kinase signaling. a – b Hierarchical clustering analysis ( a ) and GO analysis ( b ) of microarray data for HCC cells overexpressing uc.134 and control cells. Upregulated genes are highlighted in yellow , and downregulated genes are in blue . (fold changes >3; P < 0.05). The top ten most significantly enriched GO terms in differentially expressed genes relative to all other genes in the genome are shown. c IP experiment. d The overexpression of uc.134 prolonged the half-life of LATS1. Immunoblot detection of LATS1 ( left ); IB data were quantified using the ImageJ software ( right ). Error bars indicate the mean ± SD. ** P < 0.01. e Ubiquitination assays of cells transfected with uc.134 plasmid in Bel7402 cells ( left ) or siRNA in QGY7701 cells ( right ). The bottom panels depict the input of the cell lysates. f Western blotting in Bel7402 cells showed that uc.134 reversed the CUL4A-mediated inhibition of Hippo kinase activity. g Western blots showed that the overexpression of uc.134 activates Hippo kinase signaling ( left ), whereas silencing of uc.134 yielded opposite results ( right ). h qRT-PCR analysis of cells overexpressing uc.134 or co-transfected with YAP plasmids compared with vector controls. Experiments were performed in triplicate, and data are presented as the mean ± SD. * P < 0.05; ** P < 0.01; and *** P < 0.001

Article Snippet: Sample labeling and array hybridization were performed according to the Arraystar microarray-based gene expression analysis protocol (Arraystar, Rockville, MD).

Techniques: Microarray, Over Expression, Western Blot, Software, Transfection, Plasmid Preparation, Inhibition, Activity Assay, Quantitative RT-PCR